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Testing Ayurvedic Ingredients for Pet Products: Heavy Metals, Pesticides and More

Writer: Care Team
Care Team
Oct 7
3 min read

Updated: 1 day ago

By the IndoFolk Wellness Care Team | Last reviewed September 2026


Quick answer: Every Ayurvedic ingredient for a pet product should be tested for identity, active content, heavy metals, pesticide residues, microbial contamination and, where relevant, aflatoxins and residual solvents. Ask for a certificate of analysis for each lot that states the method, limit and result for every test, and check results against your specification before the material is used.

Table of the seven quality tests for Ayurvedic and herbal ingredients used in pet products: identity, assay of actives, heavy metals, pesticide residues, microbial limits, aflatoxins and residual solvents, with what each checks and the typical method
Seven tests for Ayurvedic ingredients, and how to read the certificate of analysis.


Plants take up what is in the soil, water and air around them. They are dried in the open, stored, moved and sometimes mixed. That is why botanical ingredients need more testing than most synthetic ones, and why a brand's reputation can depend on a test report it never reads. Here is what to look for.


Why Herbal Ingredients Carry Extra Risk

  • Heavy metals absorbed from soil and water

  • Pesticide residues from cultivation

  • Microbes and moulds picked up during drying and storage

  • Substitution or adulteration with cheaper, similar-looking plants

  • Natural variation in active compounds between harvests and regions


The Tests Every Ingredient Should Pass

1. Identity. Confirms it is the right plant and part. Methods include visual and microscopic examination and chemical fingerprinting such as HPTLC, and sometimes DNA-based methods.

2. Assay of actives. Measures the marker compounds for standardised extracts, such as boswellic acids, curcuminoids or withanolides, usually by HPLC. The method matters, because different methods can give different numbers.

3. Heavy metals. Lead, arsenic, cadmium and mercury, typically measured by ICP-MS.

4. Pesticide residues. A multi-residue screen covering the pesticides relevant to the crop and your target markets.

5. Microbial limits. Total aerobic count, yeast and mould, and absence of pathogens such as Salmonella and E. coli.

6. Aflatoxins, where relevant. Important for some roots, seeds and dried materials prone to mould.

7. Residual solvents and moisture. For extracts, confirms extraction solvents have been removed; moisture affects stability and microbial growth.


How to Read a COA, Line by Line

  • Header: product name, botanical name, lot number, manufacturing and retest or expiry dates. The lot number must match the material you receive.

  • Each test line: the test, the method, the specification limit, the result and the units. A result with no method or limit tells you very little.

  • Conclusion and sign-off: a clear pass statement, the name of the responsible person and the lab. Note whether the lab is in-house or independent.

Watch for COAs that show only "complies" without numbers, results that are identical across different lots, or tests that are missing for the ingredient type.


Setting Your Limits

Acceptable limits depend on the ingredient, the dose in the finished product and the rules of each market you sell in. Set your specification with your formulator and regulatory adviser, often referring to pharmacopoeial or regulatory limits, and write it into your supply agreement so every lot is judged against the same standard.


Keeping Quality Consistent

  • Approve suppliers and audit them, rather than buying spot lots

  • Keep retained samples of each lot

  • Occasionally send a sample to an independent lab to confirm results

  • Test the finished product as well as the raw materials


Frequently Asked Questions

Why do Ayurvedic ingredients need heavy metal testing?

Plants can absorb heavy metals such as lead, arsenic, cadmium and mercury from soil and water, and levels vary by region and harvest. Testing every lot confirms the ingredient is within the limits set for your product and markets.

What should a herbal ingredient COA include?

A herbal ingredient COA should include the botanical name, lot number and dates, and results for identity, active content, heavy metals, pesticide residues, microbial limits and, where relevant, aflatoxins and residual solvents, each with the test method, limit and units, plus a signed conclusion.

How can I tell if a herbal extract is adulterated?

Identity testing such as HPTLC fingerprinting, compared against a reference standard, is the main way to detect substitution or adulteration. Assay results that look unusually high, or identical across lots, are also warning signs worth checking with an independent lab.


See a Sample COA

Ask us for a sample certificate of analysis for any ingredient you are considering, and we'll walk you through it.



The IndoFolk Wellness 8-step sourcing process from India: understanding your needs, selecting suppliers, sampling and feedback, finalising orders, overseeing production, final inspection, shipping and export, and delivery. Registered with COI, IEC, MSME and Startup India. Contact +91 9977313509, divyanshu@indofolkwellness.com

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